Pannier Lab
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Pannier Lab Group

The long-term goal of the Pannier Lab is to understand and design innovative biomaterials and gene delivery systems to advance biotechnology, diagnostics, fundamental understanding of embryology and tissue development, and regenerative medicine therapies. Research projects within the Pannier Lab are focused in three different themes including nonviral gene delivery, tissue engineering, and protein-cell-biomaterial interactions.

Within the nonviral gene delivery theme, our aim is to determine and understand the mechanisms that render cells responsive to the transfer of genetic material (e.g. DNA), concentrating on the cell microenvironment, the interaction between cells and biomaterials, and the intracellular processes and subsequent signaling involved during nonviral gene delivery. Within the tissue engineering theme, our objective is to develop biomaterial scaffolds and culture systems to understand and promote tissue, organ, and organism development, regeneration, and growth. Within the protein-cell-material interaction theme, projects aim to make use of a novel surface topographies, chemistries, and processing techniques and explore how these surface features affect cellular features, including adhesion, proliferation, podia indication, stem cell differentiation, and responsiveness to gene transfer.

The experimental approaches developed in the Pannier Lab provide valuable insights to the fields of gene delivery and biomaterials, with very promising potential for future scientific discovery and translation to therapeutic, biotechnological, and tissue engineering applications.

Innovation in the Lab: Engineering New Ways to Treat Disease (Faculty 101 Podcast)



Sports Nightly interview with Ronnie Green and Dr. Pannier




Dr. Pannier was awarded the Presidential Early Career Award for scientists and engineers!

Dr. Eric Farris successfully defended his dissertation on "Development, Optimization and Validation of a Chitosan-Zein Dual-Material Non Viral Gene Delivery System for Applications in Oral Gene Delivery" in April 2019

Dr. Pannier received the Holling Family Master Teacher Award/UNL University-Wide Teaching Award

Dr. Pannier was selected as co-chair to lead N150 strategic planning

Lily Foley was awarded Nebraska INBRE

Amy Mantz (with Eric Farris and Tyler Kozisek) was published in Frontiers in Chemistry

Andrew Hamann and Albert Nguyen were published in the Journal of Biological Engineering

Amy Mantz was published in Experimental Biology and Medicine

Andrew Hamann (with Kelly Broad and Albert Nguyen) was published in Biotechnology and Bioengineering



Mantz Amy, Rosenthal Alice, Farris Eric, Kozisek Tyler, Bittrich Eva, Nazari Saghar, Schubert Eva, Schubert Mathias, Stamm Manfred, Uhlmann Petra, and Pannier Angela K. Free polyethylenimine enhances substrate-mediated gene delivery on titanium substrates modified with RGD-functionalized poly(acrylic acid) brushes. Frontiers in Chemistry. 2019 Feb, 7:51.

Hamann Andrew, Nguyen Albert, and Pannier Angela K. Nucleic acid delivery to mesenchymal stem cells: a review of nonviral methods and applications. Journal of Biological Engineering. 2019 Jan, 13:7.

Mantz Amy and Pannier Angela K. Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery Experimental Biology and Medicine. 2019 Jan, 244(2), 100-113.

Hamann Andrew, Broad Kelly, Nguyen Albert, and Pannier Angela K. Mechanisms of unprimed and dexamethasone-primed nonviral gene delivery to human mesenchymal stem cells. Biotechnology and Bioengineering. 2019, 116(2) 427-443.

Rosenthal Alice, Mantz Amy, Nguyen Albert, Bittrich Eva, Schubert Eva, Schubert Mathias, Stamm Manfred, Pannier Angela K., and Uhlmann Petra. Biofunctionalization of Titanium Substrates Using Nanoscale Polymer Brushes with Cell Adhesion Peptides J. Phys. Chem. B, 2018, 122, 25, 6543-6550.

Erickson, Alek G., Laughlin Taylor D., Romereim Sarah M., Sargus-Patino Catherine N., Pannier Angela K., and Dudley Andrew T. A Tunable, Three-Dimensional In Vitro Culture Model of Growth Plate Cartilage Using Alginate Hydrogel Scaffolds Tissue Engineering
Part A
. 2018 Jan; 24(1-2): 94-105.