Publications

• Walsh SC, Miles JR, Broeckling CD, Rempel LA, Wright-Johnson EC, Pannier AK. 2023. Secreted metabolome of porcine blastocysts encapsulated within in vitro 3D alginate hydrogel culture systems undergoing morphological changes provides insights into specific mechanisms involved in the initiation of porcine conceptus elongation. Reproduction, Fertility and Development. 35(5), 375-394.
• Kozisek T, Samuelson L, Hamann A, Pannier AK. 2023. Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells. Journal of Biological Engineering. 17:76.
• Miles JR, Walsh SC, Rempel LA, Pannier AK. 2022. Mechanisms regulating the initiation of porcine conceptus elongation. Molecular Reproduction & Development. 
• Walsh SC, Miler JR, Keel BN, Rempel LA, Wright-Johnson EC, Lindholm-Perry AK, Oliver WT, Pannier AK. 2022. Global analysis of differential gene expression within the porcine conceptus transcriptome as it transitions through spherical, ovoid, and tubular morphologies during the initiation of elongation. Molecular Reproduction & Development. 89(4), 175-201.
• Kozisek T, Hamann A, Samuelson L, Fudolig M, Pannier AK. 2021. Comparison of promoter, DNA vector, and cationic carrier for efficient transfection of hMSCs from multiple donors and tissue sources. Molecular Therapy Nucleic Acids. 26, 81-93.
• Lampe AT, Farris EJ, Brown DM, Pannier AK. 2021. High- and low-molecular-weight chitosan act as adjuvants during single-dose influenza A virus protein vaccination through distinct mechanisms. Biotechnology and Bioengineering. 118(3), 1224-1243.
• Hamann A, Kozisek T, Broad K, Pannier AK. 2020. Glucocorticoid Priming of Nonviral Gene Delivery to hMSCs Increases Transfection by Reducing Induced Stresses. Molecular Therapy Methods & Clinical Development. 18, 713-722.
• Lampe AT, Puniya BL, Pannier AK, Helikar T, Brown DM. 2020. Combined TLR4 and TLR9 agonists induce distinct phenotypic changes in innate immunity in vitro and in vivo. Cellular Immunology. 355, 104149.
• Kozisek T, Hamann A, Nguyen A, Miller M, Plautz S, Pannier AK. 2020. High-throughput screening of clinically approved drugs that prime nonviral gene delivery to human Mesenchymal stem cells. Journal of Biological Engineering. 14, 16.
• Hamann A, Thomas AK, Kozisek T, Farris E, Luck S, Zhang Y, Pannier AK. Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection. Experimental Biology and Medicine. 245(7), 606-619. 
• Walsh SC, Miles JR, Yao L, Broeckling CD, Rempel LA, Wright-Johnson EC, Pannier AK. 2020. Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation. Molecular Reproduction and Development. 87(1), 174-190. 
• Farris E, Sanderfer K, Lampe A, Brown DM, Ramer-Tait AE, Pannier AK. 2018. Oral Non-Viral Gene Delivery for Applications in DNA Vaccination and Gene Therapy. Current Opinion in Biomedical Engineering. 7, 51-57.
• Mantz Amy, Rosenthal Alice, Farris Eric, Kozisek Tyler, Bittrich Eva, Nazari Saghar, Schubert Eva, Schubert Mathias, Stamm Manfred, Uhlmann Petra, and Pannier Angela K. 2019. Free polyethylenimine enhances substrate-mediated gene delivery on titanium substrates modified with RGD-functionalized poly(acrylic acid) brushes. Frontiers in Chemistry. 7, 51.
• Hamann A, Nguyen A, Pannier AK. 2019. Nucleic acid delivery to mesenchymal stem cells: a review of nonviral methods and applications. Journal of Biological Engineering. 13, 7.
• Mantz A and Pannier AK. 2019. Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery. Experimental Biology and Medicine. 244(2), 100-113.
• Hamann A, Broad K, Nguyen A, Pannier AK. 2019. Mechanisms of unprimed and dexamethasone-primed nonviral gene delivery to human mesenchymal stem cells. Biotechnology and Bioengineering. 116(2), 427-443.
• Rosenthal A, Mantz A, Nguyen A, Bittrich E, Schubert E, Shubert M, Stamm M, Pannier AK, Uhlmann P. 2018. Biofunctionalization of titanium substrates using nanoscale polymer brushes with cell adhesion peptides. The Journal of Physical Chemistry B. 122(25), 6543-6550.
• Erikson AG, Laughlin TD, Romereim SM, Sargus-Patino CN, Pannier AK, Dudley AT. 2018. A tunable three-dimensional in vitro culture model of growth plate cartilage using alginate hydrogel scaffolds. Tissue Engineering Part A. 24(1-2), 94-105.
• Laughlin TD, Miles JR, Wright-Johnson EC, Rempel LA, Lents CA, Pannier AK. 2017. Development of pre-implantation porcine blastocysts cultured within alginate hydrogel systems either supplemented with secreted phosphoroprotein 1 or conjugated with Arg-Gly-Asp peptide. Reproduction, Fertility and Development. 29(12):2345-2356.
• Miles JR, Laughlin TD, Sargus-Patino CN, Pannier AK. 2017. In vitro porcine blastocyst development in three-dimensional alginate hydrogels. Molecular Reproduction and Development. 84(9), 775-787.
• Farris E, Brown DM, Ramer-Tait AE, Pannier AK. 2017. Chitosan-zein nano-in-microparticles capable of mediating in vivo transgene expression following oral delivery. Journal of Controlled Release. 249, 150-161.
• Nguyen A, Beyersdorf J, Riethoven J, Pannier AK. 2016. High-throughput screening of clinically approved drugs that prime polyethylenimine transfection reveals modulation of mitochondria dysfunction response improves gene transfer efficiencies. Bioengineering & Translatinal Medicine. 1(2), 123-135.
• Farris E, Brown DM, Ramer-Tait AE, Pannier AK. 2016. Micro- and nanoparticles for DNA vaccine delivery. Experimental Biology and Medicine. 241(9), 919-929.
• Kelly AM, Plautz SA, Zempleni J, Pannier AK. 2016. Glucocorticoid cell priming enhances transfection outcomes in adult human mesenchymal stem cells. Molecular Therapy. 24(2), 331-341.
• Peev D, Hofmann T, Kananizedeh N, Beeram S, Rodriguez E, Wilmer S, Rodenhausen KB, Herzinger CM, Kaspustis T, Pfaunmiller E, Nguyen A, Korlacki R, Pannier AK, Schubert E, Hage D, Schubert M. 2016. Anisotropic contrast optical microscope. Review of Scientific Instruments. 87(11).
• Kasputis T, Pieper A, Rodenhausen KB, Schmidt D, Sekora D, Rice C, Franke-Schubert E, Schubert M, Pannier AK. 2015. Use of precisely-sculptured thin film (STF) substrates with generalized ellipsometry to determine spatial distribution of adsorbed fibronectin to nanostructured columnar topographies and effect on cell adhesion. Acta Biomaterialia. 18, 88-99.
• Martin TM, Wysocki BJ, Wysocki TA, Pannier AK. 2015. Identifying intracellular pDNA losses from a model of nonviral gene delivery. IEEE Transactions on NanoBioscience. 14(4), 455-464.
• Martin TM, Plautz SA, Pannier AK. 2015. Temporal endogenous gene expression profiles in response to lipid-mediated transfection. The Journal of Gene Medicine. 17(1-2), 14-32.
• Martin TM, Plautz SA, Pannier AK. 2015. Temporal endogenous gene expression profiles in response to polymer-mediated transfection and profile comparison to lipid-mediated transfection. The Journal of Gene Medicine. 17(1-2), 33-53.
• Koenig M, Kasputis T, Schmidt D, Rodenhausen KB, Eichhorn KJ, Pannier AK, Schubert M, Stamm M, Uhlmann P. 2014. Combined QCM-D/GE as a tool to characterize stimuli-responsive swelling of and protein adsorption on polymer brushes grafted on 3D-nanostructures. Analytical and Bioanalytical Chemistry. 406(28), 7233-7242.
• Wysocki BJ, Martin TM, Wysocki TA, Pannier AK. 2014. Simulation supported estimation of end-to-end transmission parameters in non-viral gene delivery. IEEE International Conference on Communication. 4179-4183.
• Sargus-Patino CN, Wright EC, Plautz SA, Miles JR, Vallet JL, Pannier AK. 2013. In vitro development of pre-implantation porcine embryos using alginate hydrogels as a three-dimensional matrix. Reproduction, Fertility, and Development. 26(7), 943-953.
• Martin TM, Wysocki BJ, Beyersdorf JP, Wysocki TA, Pannier AK. 2014. Integrating mitosis, toxicity, and transgene expression in a telecommunications packet-switched network model of lipoplex-mediated gene delivery. Biotechnology and Bioengineering. 111(8), 1659-1671.
• Kasputis T, Pieper A, Schubert M, Pannier AK. 2014. Dynamic analysis of DNA nanoparticle immobilization to model biomaterial substrates using combinatorial spectroscopic ellipsometry and quartz crystal microbalance with dissipation. Thin Solid Films: ICSE-VI Proceedings. 571(3), 637-643.
• Martin TM, Plautz SA, Pannier AK. 2013. Network analysis of endogenous gene expression profiles after polyethyleneimine-mediated DNA delivery. The Journal of Gene Medicine. 15(3-4), 142-154.
• Wysocki BJ, Wysocki TA, Martin TM, Pannier AK. 2013. Modeling nonviral gene delivery as a macro-to-nano communication system. Nano Communication Networks. 4(1), 14-22.
• Kasputis T, Koenig M, Schmidt D, Sekora D, Rodenhausen KB, Eichhorn KJ, Uhlmann P, Schubert E, Pannier AK, Schubert M, Stamm M. 2013. Slanted columnar thin films prepared by glancing angle deposition functionalized with polyacrylic acid Guiselin polymer brushes. Journal of Physical Chemistry, Part C. 117(27), 13971-13980.
• Pannier AK, Segura T. 2013. Surface- and hydrogel-mediated delivery of nucleic acid nanoparticles. Methods in Molecular Biology. 948, 149-169.
• Kelly AM, Lammers A, Jones DD, Stowell R, Hoy R, Curtis E, Pannier AK. 2013. Implementation of a "Rapid Design Challenge" in a cross-disciplinary senior capstone course and evaluation of device performance. American Society for Engineering Education Conference Proceedings. ID 6997.
• Regier MC, Taylor JD, Borcyk T, Yang Y, Pannier AK. 2012. Fabrication and characterization of DNA-loaded zein nanospheres. Journal of Nanobiotechnology. 10(44).
• Kasputis T and Pannier AK. 2012. The role of surface chemistry-induced cell characteristics on nonviral gene delivery to mouse fibroblasts. Journal of Biological Engineering. 6(1), 17.
• Othman SF, Curtis ET, Plautz SA, Pannier AK, Butler SD, Xu H. 2012. MR elastography monitoring of tissue-engineered constructs. NMR Biomedicine. 25(3), 452-463.
• Rodenhausen KB, Schmidt D, Kasputis T, Pannier AK, Schubert E, Schubert M. 2012. Generalized ellipsometry in-situ quantification of organic adsorbate attachment within slanted columnar thin films. Optics Express. 20(5), 5419-5428.
• Sharp AT, Pannier AK, Wysocki BJ, Wysocki TA. 2012. A novel telecommunications-base approach to HIV modeling and simulation. Nano Communication Networks. 3(2), 129-137.
• Plautz SA, Boanca G, Riethoven J-JM, Pannier AK. 2011. Microarray analysis of gene expression profiles in cells transfected with nonviral vectors. Molecular Therapy. 19(12), 2144-2151.
• Rodenhausen KB, Kasputis T, Pannier AK, Gerasimov JY, Lai RY, Solinsky M, Tiwalk TE, Wang H, Sarkar A, Hofmann T, Ianno N, Schubert M. 2011. Combined optical and acoustical method for determination of thickness and porosity of transparent organic layers below the ultra-thin film limit. Review of Scientific Instruments. 82(10), 103-111.
• Singh D, Pannier AK, Zempleni J. 2011. Identification of holocarboxylase synthetase chromatin binding sites in human mammary cell lines using the DNA adenine methyltransferase identification technology. Analytical Biochemistry. 413(1), 55-59.
• Bellis AD, Penalver-Bernabe B, Weiss MS, Yarrington ME, Barbolina MV, Pannier AK, Jeruss JS, Broadbelt LJ, Shea LD. 2010. Cellular arrays for large-scale analysis of transcription factor activity. Biotechnology and Bioengineering. 108(2), 395-403.
• Rodenhausen KB, Duensing BA, Kasputis T, Pannier AK, Hofmann T, Schubert M, Tiwald TE, Solinsky M, Wagner M. 2011. In-situ monitoring of alkanethiol self-assembled monolayer chemisorption with combined spectroscopic ellipsometry and quartz crystal microbalance techniques. Thin Solid Films. 519(9), 2817-2820.
• Pannier AK, Wieland JA, Shea LD. 2008. Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes. Acta Biomaterilia. (4)1, 26-39.
• Pannier AK, Ariazi EA, Bellis AD, Bengali Z, Jordan VC, Shea LD. 2007. Bioluminescence imaging for assessment and normalization in transfected cell arrays. Biotechnology and Bioengineering. 98, 486-497.
• Pannier AK, Anderson BC, Shea LD. 2005. Substrate-mediated delivery from self-assembled monolayers: effect of surface ionization, hydrophilicity, and patterning. Acta Biomaterialia. 1(5), 511-522.
• Bengali Z, Pannier AK, Segura T, Anderson BC, Jang JH, Mustoe TA, Shea LD. 2005. Gene delivery through cell culture substrate adsorbed DNA complexes. Biotechnology and Bioengineering. 90(3), 290-302.
• Pannier AK, Shea LD. 2004. Controlled release systems for DNA delivery. Molecular Therapy. 10(1), 19-26.
• Pannier AK, Arora V, Iversen PL, Brand RM. 2004. Transdermal delivery of phosphorodiamidate Morpholino oligomers across hairless mouse skin. International Journal of Pharmaceutics. 275(1-2), 217-226.
• Pannier AK, Shea LD. 2004. Controlled-release systems for non-viral vectors. The Journal of the Robert H. Lurie Comprehensive Cancer Center of Northwestern University. 9, 35-39.
• Pannier AK, Brand RM, Jones DD. 2003. Fuzzy modeling of skin permeability coefficients. Pharmaceutical Research. 20, 143-148.

Book Chapters

• Heck, K, Farris E, Pannier AK. 2024. Formulation of chitosan-zein nano-in-microparticles for oral DNA delivery. Methods in Molecular Biology. Vol 2720, 165-176.
• Pannier AK, Kozisek T, Segura T. 2019. Surface- and hydrogel-mediated delivery of nucleic acid nanoparticles. Nanotechnology for Nucleic Acid Delivery. 1943, 177-197.
• Martin TM and Pannier AK. 2016. Molecular mechanisms of nonviral gene delivery. The World Scientific Encyclopedia of Nanomedicine and Bioengineering I. 141-173.
• Kasputis T, Farris E, Guerreiro GV, Taylor J, Pannier AK. 2016. Substrate-mediated gene delivery. The World Scientific Encyclopedia of Nanomedicine and Bioengineering I. 219-260.
• Pannier AK and Segura T. 2013. Surface- and hydrogel-mediated delivery of nucleic acid nanoparticles. Nanotechnology for Nucleic Acid Delivery: Methods in Molecular Biology. 948, 149-169.

Patents

• Hofmann T, Schubert MM, Kasputis T, Pannier AK, Herzinger CM, Woollam JA. Method of obtaining micrographs of transparent or semi-transparent specimens using anisotropic contrast. U.S. Patent No. 10,026,167. July 17, 2018.
• Segura T, Shea LD, Pannier AK, Bengali Z, Jang JH, Chung P, Anderson B. Controlled surface-associated delivery of genes and oligonucleotides. U.S. Patent No. 7,029,697. April 18, 2006.
• Pannier AK, Ariazi EA, Jordan VC, Shea LD. Cellular arrays. U.S. Patent Application: 11/809,772. Filing date: June 1, 2007.